Coding

Part:BBa_K3757002:Design

Designed by: Catia Harnack   Group: iGEM21_Tuebingen   (2021-09-25)


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A His 6 Tag was embedded in loop 5 of the cyclotide. Including Esp3I Typ IIS recognition sites.


Source

From Momordica cochinchinensis. Amino acid sequence from Cybase (http://www.cybase.org.au/index.php?page=card&table=protein&id=3) reverse transciped into dna sequence with codon usage optimized for Nicotiana bethaniana.

References