Coding
Part:BBa_K3757002:Design
Designed by: Catia Harnack Group: iGEM21_Tuebingen (2021-09-25)
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Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A His 6 Tag was embedded in loop 5 of the cyclotide. Including Esp3I Typ IIS recognition sites.
Source
From Momordica cochinchinensis. Amino acid sequence from Cybase (http://www.cybase.org.au/index.php?page=card&table=protein&id=3) reverse transciped into dna sequence with codon usage optimized for Nicotiana bethaniana.